members.dirk schubert | ||||||||||||||||||||||||||||
Personal website |
||||||||||||||||||||||||||||
profile | top | |||||||||||||||||||||||||||
Current position : Birthplace: Alma mater: Fields of study: |
Assistant professor Heinrich-Heine-University, Düsseldorf (Dipl., Ph.D.) Biology, Natural Sciences |
|||||||||||||||||||||||||||
research interests | top | |||||||||||||||||||||||||||
Methodological expertise |
||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||
On
the one hand, visually identified neurons are characterized electrophysiologically
and, with the help of intracellular biocytin staining, morphologically
on a quantitative basis. On the other hand, local photolytic release of
an excitatory neurotransmitter (L-glutamate) is used to investigate an
larger area of the barrel cortex, covering all cortical layers and two
barrel-associated functional columns, for neurons sending synaptic inputs
onto the recorded neuron. Thus, for each recorded cell we obtain detailed
maps of its functional, monosynaptic input-connectivity. |
||||||||||||||||||||||||||||
In acute brain slice preparations (background) visually identified neurons are electro-physiologically characterized. After that, inactive caged-glutamate is added to the bath solution. A UV flash is focused through the objective onto numerous different cortical fields of 50x50 µm in size. In these fields, very locally, caged glutamate is photolysed to active L-glutamate which activates local neurons. With computer controlled methods a large cortical area (grid) is mapped without gaps for any origins of monosynaptic excitatory and inhibitory inputs. The spatial resolution is sufficient to reveal not only layer-, but also sublayer-specific differences in functional connectivity. |
||||||||||||||||||||||||||||
In the last recent years the combination of whole-cell recording and caged-glutamate photolysis revealed new, sometimes even surprising details about the integration of defined neuron-classes in the somatosensory cortex. For several neuron classes we could give evidence for cell-type as well as layer-specific properties that revealed intracortical translaminar and transcolumnar pathways which partially so far have been assumed without direct prove only. These data delivered important details for a better understanding of the modular organization of cortical processsing of sensory information. Recently the method described before was further improved by adding the technique of simultaneous recording of pairs neurons. Mapping the functional connectivity of two synaptically connected neurons simultaneously hopefully will give new insights about, how cortical microcircuits integrate sensory information. |
||||||||||||||||||||||||||||
Functional connectivity maps of two synaptically connected pyramidal cells in layers II/III of the rat barrel cortex: Photomicrograph of the native brain slice with positioned recording electrodes (center) and, respectively, drawings of their columnar and laminar organization (left/rightp) are super-imposed by the somatodendritic reconstructions of each of the two simultaneously recorded neurons and the maps of origins for synaptic inputs. The maps illustrate the origins for excitatory (green to red) inhibitory synaptic inputs (blue) onto each of the pyramidal cells. |
||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||
The bewildering complexity of cortical microcircuits at the single cell level gives rise to surprisingly robust emergent activity patterns at the level of laminar and columnar local field potentials (LFPs) in response to targeted local stimuli. Simultaneous multi-site recordings using micro-electrode array chips are well suited for investigation of the microcircuity of rat barrel cortex because of a high repeatability of stimulus-induced responses, and typical spatial distributions of LFP responses to stimuli in supragranular, granular, and infragranular layers. |
||||||||||||||||||||||||||||
Upper left: Photomicrograph of slice preparation of the barrel cortex mounted on top of a MEA chip with a patch clamp recording electrode positioned in the granular layer IV. White asterisks mark the positions of the barrels in layer IV. wm - white matter. Lower left: Stimulus responses recorded from the 60 channel MEA chip with electrodes aligned in an 8x8 grid Stimuli were applied at an electrode in layers II/III. Right column: Responses upon supra-granular stimulation. The stimulated row is indicated by the diamond symbol at time zero. Voltage signal are expressed in colour. The first row contains the original local field potential data, the second row contains the residuals, and the thrid row contains the one-dimensional current source density (CSD) estimates. |
||||||||||||||||||||||||||||
curriculum vitae | top | |||||||||||||||||||||||||||
Research Career | ||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||
Teaching | ||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||
top | ||||||||||||||||||||||||||||
|
||||||||||||||||||||||||||||